6T1F

Crystal structure of the C-terminally truncated chromosome-partitioning protein ParB from Caulobacter crescentus complexed to the centromeric parS site

Summary for 6T1F

• Entry DOI: 10.2210/pdb6t1f/pdb
• Descriptor: Chromosome-partitioning protein ParB, DNA (5'-D(*GP*GP*AP*TP*GP*TP*TP*TP*CP*AP*CP*GP*TP*GP*AP*AP*AP*CP*AP*TP*CP*C)-3') (2 entities in total)
• Functional Keywords: chromosome segregation, chromosome maintenance, protein-dna recognition, dna binding protein
• Biological source: Caulobacter vibrioides (strain NA1000 / CB15N); More
• Total number of polymer chains: 8
• Total formula weight: 139104.96

Authors

Jalal, A.S.B.,Pastrana, C.L.,Tran, N.T.,Stevenson, C.E.M.,Lawson, D.M.,Moreno-Herrero, F.,Le, T.B.K. (deposition date: 2019-10-04, release date: 2020-10-14, Last modification date: 2024-01-24)

Primary citation

Jalal, A.S.,Tran, N.T.,Stevenson, C.E.,Chimthanawala, A.,Badrinarayanan, A.,Lawson, D.M.,Le, T.B.
A CTP-dependent gating mechanism enables ParB spreading on DNA.
Elife, 10:-, 2021

PubMed Abstract: Proper chromosome segregation is essential in all living organisms. The ParA-ParB- system is widely employed for chromosome segregation in bacteria. Previously, we showed that ParB requires cytidine triphosphate to escape the nucleation site and spread by sliding to the neighboring DNA (Jalal et al., 2020). Here, we provide the structural basis for this transition from nucleation to spreading by solving co-crystal structures of a C-terminal domain truncated ParB with and with a CTP analog. Nucleating ParB is an open clamp, in which is captured at the DNA-binding domain (the DNA-gate). Upon binding CTP, the N-terminal domain (NTD) self-dimerizes to close the NTD-gate of the clamp. The DNA-gate also closes, thus driving into a compartment between the DNA-gate and the C-terminal domain. CTP hydrolysis and/or the release of hydrolytic products are likely associated with reopening of the gates to release DNA and recycle ParB. Overall, we suggest a CTP-operated gating mechanism that regulates ParB nucleation, spreading, and recycling.

PubMed: 34397383
DOI: 10.7554/eLife.69676

Experimental method

X-RAY DIFFRACTION (2.9 Å)