6T14

Native structure of mosquitocidal Cyt1A protoxin obtained by Serial Femtosecond Crystallography on in vivo grown crystals at pH 7

これはPDB形式変換不可エントリーです。

6T14 の概要

• エントリーDOI: 10.2210/pdb6t14/pdb
• 分子名称: Type-1Aa cytolytic delta-endotoxin, SODIUM ION (3 entities in total)
• 機能のキーワード: mosquitocidal toxin, in vivo grown nanocrystals, toxin
• 由来する生物種: Bacillus thuringiensis subsp. israelensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27377.95

構造登録者

Tetreau, G.,Banneville, A.S.,Andreeva, E.,Brewster, A.S.,Hunter, M.S.,Sierra, R.G.,Young, I.D.,Boutet, S.,Coquelle, N.,Cascio, D.,Sawaya, M.R.,Sauter, N.K.,Colletier, J.P. (登録日: 2019-10-03, 公開日: 2020-10-14, 最終更新日: 2024-10-23)

主引用文献

Tetreau, G.,Banneville, A.S.,Andreeva, E.A.,Brewster, A.S.,Hunter, M.S.,Sierra, R.G.,Teulon, J.M.,Young, I.D.,Burke, N.,Grunewald, T.A.,Beaudouin, J.,Snigireva, I.,Fernandez-Luna, M.T.,Burt, A.,Park, H.W.,Signor, L.,Bafna, J.A.,Sadir, R.,Fenel, D.,Boeri-Erba, E.,Bacia, M.,Zala, N.,Laporte, F.,Despres, L.,Weik, M.,Boutet, S.,Rosenthal, M.,Coquelle, N.,Burghammer, M.,Cascio, D.,Sawaya, M.R.,Winterhalter, M.,Gratton, E.,Gutsche, I.,Federici, B.,Pellequer, J.L.,Sauter, N.K.,Colletier, J.P.
Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade.
Nat Commun, 11:1153-1153, 2020

PubMed Abstract: Cyt1Aa is the one of four crystalline protoxins produced by mosquitocidal bacterium Bacillus thuringiensis israelensis (Bti) that has been shown to delay the evolution of insect resistance in the field. Limiting our understanding of Bti efficacy and the path to improved toxicity and spectrum has been ignorance of how Cyt1Aa crystallizes in vivo and of its mechanism of toxicity. Here, we use serial femtosecond crystallography to determine the Cyt1Aa protoxin structure from sub-micron-sized crystals produced in Bti. Structures determined under various pH/redox conditions illuminate the role played by previously uncharacterized disulfide-bridge and domain-swapped interfaces from crystal formation in Bti to dissolution in the larval mosquito midgut. Biochemical, toxicological and biophysical methods enable the deconvolution of key steps in the Cyt1Aa bioactivation cascade. We additionally show that the size, shape, production yield, pH sensitivity and toxicity of Cyt1Aa crystals grown in Bti can be controlled by single atom substitution.

PubMed: 32123169
DOI: 10.1038/s41467-020-14894-w

実験手法

X-RAY DIFFRACTION (1.86 Å)