6T0W

Human Influenza B polymerase recycling complex

Summary for 6T0W

• Entry DOI: 10.2210/pdb6t0w/pdb
• EMDB information: 10361
• Descriptor: Polymerase acidic protein, RNA-directed RNA polymerase catalytic subunit, Polymerase PB2, ... (4 entities in total)
• Functional Keywords: influenza, polymerase, viral transcription, rna, viral protein
• Biological source: Influenza B virus; More
• Total number of polymer chains: 4
• Total formula weight: 273839.51

Authors

Wandzik, J.M.,Kouba, T.,Karuppasamy, M.,Cusack, S. (deposition date: 2019-10-03, release date: 2020-04-15, Last modification date: 2024-05-22)

Primary citation

Wandzik, J.M.,Kouba, T.,Karuppasamy, M.,Pflug, A.,Drncova, P.,Provaznik, J.,Azevedo, N.,Cusack, S.
A Structure-Based Model for the Complete Transcription Cycle of Influenza Polymerase.
Cell, 181:877-, 2020

PubMed Abstract: Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). Here, we visualize by cryoelectron microscopy the conformational dynamics of the polymerase during the complete transcription cycle from pre-initiation to termination, focusing on the template trajectory. After exiting the active site cavity, the template 3' extremity rebinds into a specific site on the polymerase surface. Here, it remains sequestered during all subsequent transcription steps, forcing the template to loop out as it further translocates. At termination, the strained connection between the bound template 5' end and the active site results in polyadenylation by stuttering at uridine 17. Upon product dissociation, further conformational changes release the trapped template, allowing recycling back into the pre-initiation state. Influenza polymerase thus performs transcription while tightly binding to and protecting both template ends, allowing efficient production of multiple mRNAs from a single vRNP.

PubMed: 32304664
DOI: 10.1016/j.cell.2020.03.061

Experimental method

ELECTRON MICROSCOPY (3.18 Å)