6SZS

Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis

これはPDB形式変換不可エントリーです。

6SZS の概要

• エントリーDOI: 10.2210/pdb6szs/pdb
• EMDBエントリー: 10353
• 分子名称: 50S ribosomal protein L32, 50S ribosomal protein L3, 50S ribosomal protein L4, ... (56 entities in total)
• 機能のキーワード: cryo-em, bacterial ribosome rescue, bacillus ribosome rescue factor a, bacillus subtilis peptide chain release factor 2, ribosome
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 56
• 化学式量合計: 2215581.05

構造登録者

Muller, C.,Beckert, B.,Wilson, D.N. (登録日: 2019-10-02, 公開日: 2019-12-04, 最終更新日: 2024-05-22)

主引用文献

Shimokawa-Chiba, N.,Muller, C.,Fujiwara, K.,Beckert, B.,Ito, K.,Wilson, D.N.,Chiba, S.
Release factor-dependent ribosome rescue by BrfA in the Gram-positive bacterium Bacillus subtilis.
Nat Commun, 10:5397-5397, 2019

PubMed Abstract: Rescue of the ribosomes from dead-end translation complexes, such as those on truncated (non-stop) mRNA, is essential for the cell. Whereas bacteria use trans-translation for ribosome rescue, some Gram-negative species possess alternative and release factor (RF)-dependent rescue factors, which enable an RF to catalyze stop-codon-independent polypeptide release. We now discover that the Gram-positive Bacillus subtilis has an evolutionarily distinct ribosome rescue factor named BrfA. Genetic analysis shows that B. subtilis requires the function of either trans-translation or BrfA for growth, even in the absence of proteotoxic stresses. Biochemical and cryo-electron microscopy (cryo-EM) characterization demonstrates that BrfA binds to non-stop stalled ribosomes, recruits homologous RF2, but not RF1, and induces its transition into an open active conformation. Although BrfA is distinct from E. coli ArfA, they use convergent strategies in terms of mode of action and expression regulation, indicating that many bacteria may have evolved as yet unidentified ribosome rescue systems.

PubMed: 31776341
DOI: 10.1038/s41467-019-13408-7

実験手法

ELECTRON MICROSCOPY (3.06 Å)