6SX6

Guanine-rich oligonucleotide with 5'-GC end form G-quadruplex with A(GGGG)A hexad, GCGC- and G-quartets and two symmetric GG and AA base pairs

6SX6 の概要

• エントリーDOI: 10.2210/pdb6sx6/pdb
• NMR情報: BMRB: 34436
• 分子名称: GCn (1 entity in total)
• 機能のキーワード: g-quadruplex, a(gggg)a hexad, gcgc-quartet, 5'-gc ends, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7666.98

構造登録者

Pavc, D.,Wang, B.,Spindler, L.,Drevensek-Olenik, I.,Plavec, J.,Sket, P. (登録日: 2019-09-25, 公開日: 2020-07-22, 最終更新日: 2024-05-15)

主引用文献

Pavc, D.,Wang, B.,Spindler, L.,Drevensek-Olenik, I.,Plavec, J.,Sket, P.
GC ends control topology of DNA G-quadruplexes and their cation-dependent assembly.
Nucleic Acids Res., 48:2749-2761, 2020

PubMed Abstract: GCn and GCnCG, where n = (G2AG4AG2), fold into well-defined, dimeric G-quadruplexes with unprecedented folding topologies in the presence of Na+ ions as revealed by nuclear magnetic resonance spectroscopy. Both G-quadruplexes exhibit unique combination of structural elements among which are two G-quartets, A(GGGG)A hexad and GCGC-quartet. Detailed structural characterization uncovered the crucial role of 5'-GC ends in formation of GCn and GCnCG G-quadruplexes. Folding in the presence of 15NH4+ and K+ ions leads to 3'-3' stacking of terminal G-quartets of GCn G-quadruplexes, while 3'-GC overhangs in GCnCG prevent dimerization. Results of the present study expand repertoire of possible G-quadruplex structures. This knowledge will be useful in DNA sequence design for nanotechnological applications that may require specific folding topology and multimerization properties.

PubMed: 31996902
DOI: 10.1093/nar/gkaa058

実験手法

SOLUTION NMR