6SWT

Affimer9 co-crystalised with the CH domains of alpha actinin 2.

Summary for 6SWT

• Entry DOI: 10.2210/pdb6swt/pdb
• Descriptor: Alpha-actinin-2, Affimer 9 (3 entities in total)
• Functional Keywords: alpha-actinin 2, affimer, contractile protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 39972.94

Authors

Peckham, M.,Trinh, C.H.,Rogers, B. (deposition date: 2019-09-23, release date: 2020-10-07, Last modification date: 2024-01-24)

Primary citation

Curd, A.P.,Leng, J.,Hughes, R.E.,Cleasby, A.J.,Rogers, B.,Trinh, C.H.,Baird, M.A.,Takagi, Y.,Tiede, C.,Sieben, C.,Manley, S.,Schlichthaerle, T.,Jungmann, R.,Ries, J.,Shroff, H.,Peckham, M.
Nanoscale Pattern Extraction from Relative Positions of Sparse 3D Localizations.
Nano Lett., 21:1213-1220, 2021

PubMed Abstract: Inferring the organization of fluorescently labeled nanosized structures from single molecule localization microscopy (SMLM) data, typically obscured by stochastic noise and background, remains challenging. To overcome this, we developed a method to extract high-resolution ordered features from SMLM data that requires only a low fraction of targets to be localized with high precision. First, experimentally measured localizations are analyzed to produce relative position distributions (RPDs). Next, model RPDs are constructed using hypotheses of how the molecule is organized. Finally, a statistical comparison is used to select the most likely model. This approach allows pattern recognition at sub-1% detection efficiencies for target molecules, in large and heterogeneous samples and in 2D and 3D data sets. As a proof-of-concept, we infer ultrastructure of Nup107 within the nuclear pore, DNA origami structures, and α-actinin-2 within the cardiomyocyte Z-disc and assess the quality of images of centrioles to improve the averaged single-particle reconstruction.

PubMed: 33253583
DOI: 10.1021/acs.nanolett.0c03332

Experimental method

X-RAY DIFFRACTION (1.2 Å)