6SWT

Affimer9 co-crystalised with the CH domains of alpha actinin 2.

6SWT の概要

• エントリーDOI: 10.2210/pdb6swt/pdb
• 分子名称: Alpha-actinin-2, Affimer 9 (3 entities in total)
• 機能のキーワード: alpha-actinin 2, affimer, contractile protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39972.94

構造登録者

Peckham, M.,Trinh, C.H.,Rogers, B. (登録日: 2019-09-23, 公開日: 2020-10-07, 最終更新日: 2024-01-24)

主引用文献

Curd, A.P.,Leng, J.,Hughes, R.E.,Cleasby, A.J.,Rogers, B.,Trinh, C.H.,Baird, M.A.,Takagi, Y.,Tiede, C.,Sieben, C.,Manley, S.,Schlichthaerle, T.,Jungmann, R.,Ries, J.,Shroff, H.,Peckham, M.
Nanoscale Pattern Extraction from Relative Positions of Sparse 3D Localizations.
Nano Lett., 21:1213-1220, 2021

PubMed Abstract: Inferring the organization of fluorescently labeled nanosized structures from single molecule localization microscopy (SMLM) data, typically obscured by stochastic noise and background, remains challenging. To overcome this, we developed a method to extract high-resolution ordered features from SMLM data that requires only a low fraction of targets to be localized with high precision. First, experimentally measured localizations are analyzed to produce relative position distributions (RPDs). Next, model RPDs are constructed using hypotheses of how the molecule is organized. Finally, a statistical comparison is used to select the most likely model. This approach allows pattern recognition at sub-1% detection efficiencies for target molecules, in large and heterogeneous samples and in 2D and 3D data sets. As a proof-of-concept, we infer ultrastructure of Nup107 within the nuclear pore, DNA origami structures, and α-actinin-2 within the cardiomyocyte Z-disc and assess the quality of images of centrioles to improve the averaged single-particle reconstruction.

PubMed: 33253583
DOI: 10.1021/acs.nanolett.0c03332

実験手法

X-RAY DIFFRACTION (1.2 Å)