6SW1

Crystal Structure of P. aeruginosa PqsL: R41Y, I43R, G45R, C105G mutant

6SW1 の概要

• エントリーDOI: 10.2210/pdb6sw1/pdb
• 分子名称: Probable FAD-dependent monooxygenase, FLAVIN-ADENINE DINUCLEOTIDE, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: oxidoreductase, photocatalysis, fad, nad(p), biosynthetic pathway, antibiotic
• 由来する生物種: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45320.89

構造登録者

Mattevi, A.,Rovida, S. (登録日: 2019-09-19, 公開日: 2020-06-03, 最終更新日: 2024-01-24)

主引用文献

Ernst, S.,Rovida, S.,Mattevi, A.,Fetzner, S.,Drees, S.L.
Photoinduced monooxygenation involving NAD(P)H-FAD sequential single-electron transfer.
Nat Commun, 11:2600-2600, 2020

PubMed Abstract: Light-dependent or light-stimulated catalysis provides a multitude of perspectives for implementation in technological or biomedical applications. Despite substantial progress made in the field of photobiocatalysis, the number of usable light-responsive enzymes is still very limited. Flavoproteins have exceptional potential for photocatalytic applications because the name-giving cofactor intrinsically features light-dependent reactivity, undergoing photoreduction with a variety of organic electron donors. However, in the vast majority of these enzymes, photoreactivity of the enzyme-bound flavin is limited or even suppressed. Here, we present a flavoprotein monooxygenase in which catalytic activity is controllable by blue light illumination. The reaction depends on the presence of nicotinamide nucleotide-type electron donors, which do not support the reaction in the absence of light. Employing various experimental approaches, we demonstrate that catalysis depends on a protein-mediated photoreduction of the flavin cofactor, which proceeds via a radical mechanism and a transient semiquinone intermediate.

PubMed: 32451409
DOI: 10.1038/s41467-020-16450-y

実験手法

X-RAY DIFFRACTION (1.8 Å)