6SSG

Transaminase with DCS bound

Summary for 6SSG

• Entry DOI: 10.2210/pdb6ssg/pdb
• Descriptor: ForI-DCS, [4-[(~{Z})-[(2~{R},5~{R})-5-(azanyloxymethyl)-3,6-bis(oxidanylidene)piperazin-2-yl]methoxyiminomethyl]-6-methyl-5-oxidanyl-pyridin-3-yl]methyl dihydrogen phosphate, SULFATE ION, ... (4 entities in total)
• Functional Keywords: dcs bound, transferase
• Biological source: Streptomyces kaniharaensis
• Total number of polymer chains: 2
• Total formula weight: 90553.48

Authors

Naismith, J.H.,Gao, S. (deposition date: 2019-09-06, release date: 2020-01-15, Last modification date: 2024-05-15)

Primary citation

Gao, S.,Liu, H.,de Crecy-Lagard, V.,Zhu, W.,Richards, N.G.J.,Naismith, J.H.
PMP-diketopiperazine adducts form at the active site of a PLP dependent enzyme involved in formycin biosynthesis.
Chem.Commun.(Camb.), 55:14502-14505, 2019

PubMed Abstract: ForI is a PLP-dependent enzyme from the biosynthetic pathway of the C-nucleoside antibiotic formycin. Cycloserine is thought to inhibit PLP-dependent enzymes by irreversibly forming a PMP-isoxazole. We now report that ForI forms novel PMP-diketopiperazine derivatives following incubation with both d and l cycloserine. This unexpected result suggests chemical diversity in the chemistry of cycloserine inhibition.

PubMed: 31730149
DOI: 10.1039/c9cc06975e

Experimental method

X-RAY DIFFRACTION (1.47 Å)