6SRP

X-ray pump X-ray probe on thaumatin nanocrystals: 100 fs time delay

Summary for 6SRP

• Entry DOI: 10.2210/pdb6srp/pdb
• Related: 6SRJ 6SRK 6SRL 6SRO 6SRQ
• Descriptor: Thaumatin-1, L(+)-TARTARIC ACID (3 entities in total)
• Functional Keywords: radiation damage, serial femtosecond crystallography, x-ray free-electron laser, time-resolved crystallography, plant protein
• Biological source: Thaumatococcus daniellii (Katemfe)
• Total number of polymer chains: 1
• Total formula weight: 22377.15

Authors

Kloos, M.,Gorel, A.,Nass, K. (deposition date: 2019-09-05, release date: 2020-04-22, Last modification date: 2024-01-24)

Primary citation

Nass, K.,Gorel, A.,Abdullah, M.M.,V Martin, A.,Kloos, M.,Marinelli, A.,Aquila, A.,Barends, T.R.M.,Decker, F.J.,Bruce Doak, R.,Foucar, L.,Hartmann, E.,Hilpert, M.,Hunter, M.S.,Jurek, Z.,Koglin, J.E.,Kozlov, A.,Lutman, A.A.,Kovacs, G.N.,Roome, C.M.,Shoeman, R.L.,Santra, R.,Quiney, H.M.,Ziaja, B.,Boutet, S.,Schlichting, I.
Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses.
Nat Commun, 11:1814-1814, 2020

PubMed Abstract: X-ray free-electron lasers (XFELs) enable crystallographic structure determination beyond the limitations imposed upon synchrotron measurements by radiation damage. The need for very short XFEL pulses is relieved through gating of Bragg diffraction by loss of crystalline order as damage progresses, but not if ionization events are spatially non-uniform due to underlying elemental distributions, as in biological samples. Indeed, correlated movements of iron and sulfur ions were observed in XFEL-irradiated ferredoxin microcrystals using unusually long pulses of 80 fs. Here, we report a femtosecond time-resolved X-ray pump/X-ray probe experiment on protein nanocrystals. We observe changes in the protein backbone and aromatic residues as well as disulfide bridges. Simulations show that the latter's correlated structural dynamics are much slower than expected for the predicted high atomic charge states due to significant impact of ion caging and plasma electron screening. This indicates that dense-environment effects can strongly affect local radiation damage-induced structural dynamics.

PubMed: 32286284
DOI: 10.1038/s41467-020-15610-4

Experimental method

X-RAY DIFFRACTION (2.3 Å)