6SOE

Mouse RBM20 RRM domain

6SOE の概要

• エントリーDOI: 10.2210/pdb6soe/pdb
• NMR情報: BMRB: 34429
• 分子名称: RNA-binding protein 20 (1 entity in total)
• 機能のキーワード: alternative splicing, rna binding protein
• 由来する生物種: Mus musculus (House mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12571.59

構造登録者

Mackereth, C.D.,Upadhyay, S.K. (登録日: 2019-08-29, 公開日: 2019-11-06, 最終更新日: 2024-06-19)

主引用文献

Upadhyay, S.K.,Mackereth, C.D.
Structural basis of UCUU RNA motif recognition by splicing factor RBM20.
Nucleic Acids Res., 48:4538-4550, 2020

PubMed Abstract: The vertebrate splicing factor RBM20 (RNA binding motif protein 20) regulates protein isoforms important for heart development and function, with mutations in the gene linked to cardiomyopathy. Previous studies have identified the four nucleotide RNA motif UCUU as a common element in pre-mRNA targeted by RBM20. Here, we have determined the structure of the RNA Recognition Motif (RRM) domain from mouse RBM20 bound to RNA containing a UCUU sequence. The atomic details show that the RRM domain spans a larger region than initially proposed in order to interact with the complete UCUU motif, with a well-folded C-terminal helix encoded by exon 8 critical for high affinity binding. This helix only forms upon binding RNA with the final uracil, and removing the helix reduces affinity as well as specificity. We therefore find that RBM20 uses a coupled folding-binding mechanism by the C-terminal helix to specifically recognize the UCUU RNA motif.

PubMed: 32187365
DOI: 10.1093/nar/gkaa168

実験手法

SOLUTION NMR