6SHU

Borrelia burgdorferi BmpD nucleoside binding protein bound to adenosine

6SHU の概要

• エントリーDOI: 10.2210/pdb6shu/pdb
• 分子名称: Basic membrane protein D, ADENOSINE, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: transporter, nucleoside, purine, transport protein
• 由来する生物種: Borrelia burgdorferi (strain JD1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39617.91

構造登録者

Guedez, G.,Astrand, M.,Cuellar, J.,Hytonen, J.,Salminen, T.A. (登録日: 2019-08-08, 公開日: 2020-02-12, 最終更新日: 2024-05-15)

主引用文献

Cuellar, J.,Astrand, M.,Elovaara, H.,Pietikainen, A.,Siren, S.,Liljeblad, A.,Guedez, G.,Salminen, T.A.,Hytonen, J.
Structural and Biomolecular Analyses of Borrelia burgdorferi BmpD Reveal a Substrate-Binding Protein of an ABC-Type Nucleoside Transporter Family.
Infect.Immun., 88:-, 2020

PubMed Abstract: , the causative agent of tick-borne Lyme borreliosis (LB), has a limited metabolic capacity and needs to acquire nutrients, such as amino acids, fatty acids, and nucleic acids, from the host environment. Using X-ray crystallography, liquid chromatography-mass spectrometry, microscale thermophoresis, and cellular localization studies, we show that basic membrane protein D (BmpD) is a periplasmic substrate-binding protein of an ABC transporter system binding to purine nucleosides. Nucleosides are essential for bacterial survival in the host organism, and these studies suggest a key role for BmpD in the purine salvage pathway of Because lacks the enzymes required for purine synthesis, BmpD may play a vital role in ensuring access to the purines needed to sustain an infection in the host. Furthermore, we show that, although human LB patients develop anti-BmpD antibodies, immunization of mice with BmpD does not confer protection against infection.

PubMed: 31988175
DOI: 10.1128/IAI.00962-19

実験手法

X-RAY DIFFRACTION (1.43002869382 Å)