6SHS

Abeta fibril (Morphology I)

6SHS の概要

• エントリーDOI: 10.2210/pdb6shs/pdb
• EMDBエントリー: 10204 4864 4866
• 分子名称: Amyloid-beta precursor protein (1 entity in total)
• 機能のキーワード: fibril, beta amyloid, cryo-em, protein fibril
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 52030.22

構造登録者

Kollmer, M.,Fandrich, M. (登録日: 2019-08-08, 公開日: 2019-11-06, 最終更新日: 2024-05-22)

主引用文献

Kollmer, M.,Close, W.,Funk, L.,Rasmussen, J.,Bsoul, A.,Schierhorn, A.,Schmidt, M.,Sigurdson, C.J.,Jucker, M.,Fandrich, M.
Cryo-EM structure and polymorphism of A beta amyloid fibrils purified from Alzheimer's brain tissue.
Nat Commun, 10:4760-4760, 2019

PubMed Abstract: The formation of Aβ amyloid fibrils is a neuropathological hallmark of Alzheimer's disease and cerebral amyloid angiopathy. However, the structure of Aβ amyloid fibrils from brain tissue is poorly understood. Here we report the purification of Aβ amyloid fibrils from meningeal Alzheimer's brain tissue and their structural analysis with cryo-electron microscopy. We show that these fibrils are polymorphic but consist of similarly structured protofilaments. Brain derived Aβ amyloid fibrils are right-hand twisted and their peptide fold differs sharply from previously analyzed Aβ fibrils that were formed in vitro. These data underscore the importance to use patient-derived amyloid fibrils when investigating the structural basis of the disease.

PubMed: 31664019
DOI: 10.1038/s41467-019-12683-8

実験手法

ELECTRON MICROSCOPY (4.4 Å)