6SDW

Solution structure of Staufen1 dsRBD3+4 - hARF1 SBS dsRNA complex.

6SDW の概要

• エントリーDOI: 10.2210/pdb6sdw/pdb
• NMR情報: BMRB: 34421
• 分子名称: Double-stranded RNA-binding protein Staufen homolog 1, hARF1 SBS dsRNA (2 entities in total)
• 機能のキーワード: dsrbd-dsrna binding protein, staufen1 protein, staufen mediated mrna decay, nmr structure of rna-protein complex, rna binding protein, rna binding domain.
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30670.60

構造登録者

Yadav, D.K.,Lukavsky, P.J. (登録日: 2019-07-29, 公開日: 2020-01-15, 最終更新日: 2024-06-19)

主引用文献

Yadav, D.K.,Zigackova, D.,Zlobina, M.,Klumpler, T.,Beaumont, C.,Kubickova, M.,Vanacova, S.,Lukavsky, P.J.
Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.
Nucleic Acids Res., 48:2091-2106, 2020

PubMed Abstract: Staufen1 (STAU1) is a dsRNA binding protein mediating mRNA transport and localization, translational control and STAU1-mediated mRNA decay (SMD). The STAU1 binding site (SBS) within human ADP-ribosylation factor1 (ARF1) 3'UTR binds STAU1 and this downregulates ARF1 cytoplasmic mRNA levels by SMD. However, how STAU1 recognizes specific mRNA targets is still under debate. Our structure of the ARF1 SBS-STAU1 complex uncovers target recognition by STAU1. STAU1 dsRNA binding domain (dsRBD) 4 interacts with two pyrimidines and one purine from the minor groove side via helix α1, the β1-β2 loop anchors the dsRBD at the end of the dsRNA and lysines in helix α2 bind to the phosphodiester backbone from the major groove side. STAU1 dsRBD3 displays the same binding mode with specific recognition of one guanine base. Mutants disrupting minor groove recognition of ARF1 SBS affect in vitro binding and reduce SMD in vivo. Our data thus reveal how STAU1 recognizes minor groove features in dsRNA relevant for target selection.

PubMed: 31875226
DOI: 10.1093/nar/gkz1163

実験手法

SOLUTION NMR