6SDG

Crystal structure of the DNA binding domain of M. polymorpha Auxin Response Factor 2 (MpARF2) in complex with High Affinity DNA

6SDG の概要

• エントリーDOI: 10.2210/pdb6sdg/pdb
• 分子名称: Auxin response factor, 21-7_A, 21-7_B (3 entities in total)
• 機能のキーワード: transcription factor, dna binding, nucleus, hormone response, transcription
• 由来する生物種: Marchantia polymorpha (Liverwort); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 94276.57

構造登録者

Crespo, I.,Weijers, D.,Boer, D.R. (登録日: 2019-07-27, 公開日: 2020-04-08, 最終更新日: 2024-01-24)

主引用文献

Kato, H.,Mutte, S.K.,Suzuki, H.,Crespo, I.,Das, S.,Radoeva, T.,Fontana, M.,Yoshitake, Y.,Hainiwa, E.,van den Berg, W.,Lindhoud, S.,Ishizaki, K.,Hohlbein, J.,Borst, J.W.,Boer, D.R.,Nishihama, R.,Kohchi, T.,Weijers, D.
Design principles of a minimal auxin response system.
Nat.Plants, 6:473-482, 2020

PubMed Abstract: Auxin controls numerous growth processes in land plants through a gene expression system that modulates ARF transcription factor activity. Gene duplications in families encoding auxin response components have generated tremendous complexity in most land plants, and neofunctionalization enabled various unique response outputs during development. However, it is unclear what fundamental biochemical principles underlie this complex response system. By studying the minimal system in Marchantia polymorpha, we derive an intuitive and simple model where a single auxin-dependent A-ARF activates gene expression. It is antagonized by an auxin-independent B-ARF that represses common target genes. The expression patterns of both ARF proteins define developmental zones where auxin response is permitted, quantitatively tuned or prevented. This fundamental design probably represents the ancestral system and formed the basis for inflated, complex systems.

PubMed: 32415296
DOI: 10.1038/s41477-020-0662-y

実験手法

X-RAY DIFFRACTION (2.96 Å)