6SCO

Cryo-EM Structure of Potato Leaf Roll Virus VLP

6SCO の概要

• エントリーDOI: 10.2210/pdb6sco/pdb
• EMDBエントリー: 10142 10144
• 分子名称: Coat protein (1 entity in total)
• 機能のキーワード: luteovirus, polerovirus, vlp, capsid., virus like particle
• 由来する生物種: Potato leafroll virus (PLrV)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 69580.17

構造登録者

Byrne, M.J.,Ranson, N.A. (登録日: 2019-07-25, 公開日: 2019-10-02, 最終更新日: 2024-05-22)

主引用文献

Byrne, M.J.,Steele, J.F.C.,Hesketh, E.L.,Walden, M.,Thompson, R.F.,Lomonossoff, G.P.,Ranson, N.A.
Combining Transient Expression and Cryo-EM to Obtain High-Resolution Structures of Luteovirid Particles.
Structure, 27:1761-, 2019

PubMed Abstract: The Luteoviridae are pathogenic plant viruses responsible for significant crop losses worldwide. They infect a wide range of food crops, including cereals, legumes, cucurbits, sugar beet, sugarcane, and potato and, as such, are a major threat to global food security. Viral replication is strictly limited to the plant vasculature, and this phloem limitation, coupled with the need for aphid transmission of virus particles, has made it difficult to generate virus in the quantities needed for high-resolution structural studies. Here, we exploit recent advances in heterologous expression in plants to produce sufficient quantities of virus-like particles for structural studies. We have determined their structures to high resolution by cryoelectron microscopy, providing the molecular-level insight required to rationally interrogate luteovirid capsid formation and aphid transmission, thereby providing a platform for the development of preventive agrochemicals for this important family of plant viruses.

PubMed: 31611039
DOI: 10.1016/j.str.2019.09.010

実験手法

ELECTRON MICROSCOPY (3.3 Å)