6SCI

Structure of AdhE form 1

6SCI の概要

• エントリーDOI: 10.2210/pdb6sci/pdb
• 関連するPDBエントリー: 6SCG
• 分子名称: Aldehyde-alcohol dehydrogenase, FE (III) ION (3 entities in total)
• 機能のキーワード: aldehyde alcohol dehydrogenase, oxidoreductase
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 99901.79

構造登録者

Lovering, A.L.,Bragginton, E. (登録日: 2019-07-24, 公開日: 2020-08-26, 最終更新日: 2024-01-24)

主引用文献

Azmi, L.,Bragginton, E.C.,Cadby, I.T.,Byron, O.,Roe, A.J.,Lovering, A.L.,Gabrielsen, M.
High-resolution structure of the alcohol dehydrogenase domain of the bifunctional bacterial enzyme AdhE.
Acta Crystallogr.,Sect.F, 76:414-421, 2020

PubMed Abstract: The bifunctional alcohol/aldehyde dehydrogenase (AdhE) comprises both an N-terminal aldehyde dehydrogenase (AldDH) and a C-terminal alcohol dehydrogenase (ADH). In vivo, full-length AdhE oligomerizes into long oligomers known as spirosomes. However, structural analysis of AdhE is challenging owing to the heterogeneity of the spirosomes. Therefore, the domains of AdhE are best characterized separately. Here, the structure of ADH from the pathogenic Escherichia coli O157:H7 was determined to 1.65 Å resolution. The dimeric crystal structure was confirmed in solution by small-angle X-ray scattering.

PubMed: 32880589
DOI: 10.1107/S2053230X20010237

実験手法

X-RAY DIFFRACTION (1.95 Å)