6SBX

CdbA Form Two

6SBX の概要

• エントリーDOI: 10.2210/pdb6sbx/pdb
• 関連するPDBエントリー: 6SBW
• 分子名称: CdbA (1 entity in total)
• 機能のキーワード: nucleoid ribbon-helix-helix, dna binding protein
• 由来する生物種: Myxococcus xanthus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 23894.61

構造登録者

Lovering, A.L.,Cadby, I.T. (登録日: 2019-07-22, 公開日: 2020-04-22, 最終更新日: 2024-05-15)

主引用文献

Skotnicka, D.,Steinchen, W.,Szadkowski, D.,Cadby, I.T.,Lovering, A.L.,Bange, G.,Sogaard-Andersen, L.
CdbA is a DNA-binding protein and c-di-GMP receptor important for nucleoid organization and segregation in Myxococcus xanthus.
Nat Commun, 11:1791-1791, 2020

PubMed Abstract: Cyclic di-GMP (c-di-GMP) is a second messenger that modulates multiple responses to environmental and cellular signals in bacteria. Here we identify CdbA, a DNA-binding protein of the ribbon-helix-helix family that binds c-di-GMP in Myxococcus xanthus. CdbA is essential for viability, and its depletion causes defects in chromosome organization and segregation leading to a block in cell division. The protein binds to the M. xanthus genome at multiple sites, with moderate sequence specificity; however, its depletion causes only modest changes in transcription. The interactions of CdbA with c-di-GMP and DNA appear to be mutually exclusive and residue substitutions in CdbA regions important for c-di-GMP binding abolish binding to both c-di-GMP and DNA, rendering these protein variants non-functional in vivo. We propose that CdbA acts as a nucleoid-associated protein that contributes to chromosome organization and is modulated by c-di-GMP, thus revealing a link between c-di-GMP signaling and chromosome biology.

PubMed: 32286293
DOI: 10.1038/s41467-020-15628-8

実験手法

X-RAY DIFFRACTION (2.33 Å)