6SBW
CdbA Form One
Summary for 6SBW
| Entry DOI | 10.2210/pdb6sbw/pdb |
| Descriptor | CdbA (1 entity in total) |
| Functional Keywords | nucleoid ribbon-helix-helix, dna binding protein |
| Biological source | Myxococcus xanthus DK 1622 |
| Total number of polymer chains | 1 |
| Total formula weight | 7964.87 |
| Authors | Lovering, A.L.,Cadby, I.T. (deposition date: 2019-07-22, release date: 2020-04-22, Last modification date: 2024-05-15) |
| Primary citation | Skotnicka, D.,Steinchen, W.,Szadkowski, D.,Cadby, I.T.,Lovering, A.L.,Bange, G.,Sogaard-Andersen, L. CdbA is a DNA-binding protein and c-di-GMP receptor important for nucleoid organization and segregation in Myxococcus xanthus. Nat Commun, 11:1791-1791, 2020 Cited by PubMed Abstract: Cyclic di-GMP (c-di-GMP) is a second messenger that modulates multiple responses to environmental and cellular signals in bacteria. Here we identify CdbA, a DNA-binding protein of the ribbon-helix-helix family that binds c-di-GMP in Myxococcus xanthus. CdbA is essential for viability, and its depletion causes defects in chromosome organization and segregation leading to a block in cell division. The protein binds to the M. xanthus genome at multiple sites, with moderate sequence specificity; however, its depletion causes only modest changes in transcription. The interactions of CdbA with c-di-GMP and DNA appear to be mutually exclusive and residue substitutions in CdbA regions important for c-di-GMP binding abolish binding to both c-di-GMP and DNA, rendering these protein variants non-functional in vivo. We propose that CdbA acts as a nucleoid-associated protein that contributes to chromosome organization and is modulated by c-di-GMP, thus revealing a link between c-di-GMP signaling and chromosome biology. PubMed: 32286293DOI: 10.1038/s41467-020-15628-8 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.24 Å) |
Structure validation
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