6SA9

Endogenous Retrovirus HML2 Capsid NTD

6SA9 の概要

• エントリーDOI: 10.2210/pdb6sa9/pdb
• 分子名称: Endogenous retrovirus group K member 9 Pol protein, GLYCEROL (3 entities in total)
• 機能のキーワード: hml2 herv-k endogenous retrovirus capsid ca, virus like particle
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37412.54

構造登録者

Goldstone, D.C.,Ball, N.J.,Taylor, I.A. (登録日: 2019-07-16, 公開日: 2020-01-01, 最終更新日: 2024-11-20)

主引用文献

Acton, O.,Grant, T.,Nicastro, G.,Ball, N.J.,Goldstone, D.C.,Robertson, L.E.,Sader, K.,Nans, A.,Ramos, A.,Stoye, J.P.,Taylor, I.A.,Rosenthal, P.B.
Structural basis for Fullerene geometry in a human endogenous retrovirus capsid.
Nat Commun, 10:5822-5822, 2019

PubMed Abstract: The HML2 (HERV-K) group constitutes the most recently acquired family of human endogenous retroviruses, with many proviruses less than one million years old. Many maintain intact open reading frames and provirus expression together with HML2 particle formation are observed in early stage human embryo development and are associated with pluripotency as well as inflammatory disease, cancers and HIV-1 infection. Here, we reconstruct the core structural protein (CA) of an HML2 retrovirus, assemble particles in vitro and employ single particle cryogenic electron microscopy (cryo-EM) to determine structures of four classes of CA Fullerene shell assemblies. These icosahedral and capsular assemblies reveal at high-resolution the molecular interactions that allow CA to form both pentamers and hexamers and show how invariant pentamers and structurally plastic hexamers associate to form the unique polyhedral structures found in retroviral cores.

PubMed: 31862888
DOI: 10.1038/s41467-019-13786-y

実験手法

X-RAY DIFFRACTION (1.8 Å)