6SA3

Crystal Structure of BRD4(1) bound to inhibitor BUX4 (13)

6SA3 の概要

• エントリーDOI: 10.2210/pdb6sa3/pdb
• 分子名称: Bromodomain-containing protein 4, ~{N}-[2-methoxy-5-(4-methylpiperazin-1-yl)sulfonyl-phenyl]-3-methyl-4-oxidanylidene-5,6,7,8-tetrahydro-2~{H}-cyclohepta[c]pyrrole-1-carboxamide (3 entities in total)
• 機能のキーワード: brd4, brd4(1), inhibitor, bromodomain, epigenetic reader protein, acetylated, lysine, histone tail, protein binding-inhibitor complex, bux4, fragment, transcription
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15573.95

構造登録者

Huegle, M. (登録日: 2019-07-16, 公開日: 2020-12-09, 最終更新日: 2024-05-15)

主引用文献

Hugle, M.,Regenass, P.,Warstat, R.,Hau, M.,Schmidtkunz, K.,Lucas, X.,Wohlwend, D.,Einsle, O.,Jung, M.,Breit, B.,Gunther, S.
4-Acyl Pyrroles as Dual BET-BRD7/9 Bromodomain Inhibitors Address BETi Insensitive Human Cancer Cell Lines.
J.Med.Chem., 63:15603-15620, 2020

PubMed Abstract: Various malignant human diseases show disturbed signaling pathways due to increased activity of proteins within the epigenetic machinery. Recently, various novel inhibitors for epigenetic regulation have been introduced which promise a great therapeutic benefit. Inhibitors for the bromo- and extra-terminal domain (BET) family were of particular interest after inhibitors had shown a strong antiproliferative effect. More recently, the focus has increasingly shifted to bromodomains (BDs) outside the BET family. Based on previously developed inhibitors, we have optimized a small series of 4-acyl pyrroles, which we further analyzed by ITC, X-ray crystallography, selectivity studies, the NCI60 cell-panel, and GI determinations for several cancer cell lines. The inhibitors address both, BET and BRD7/9 BDs, with very high affinity and show a strong antiproliferative effect on various cancer cell lines that could not be observed for BD family selective inhibitors. Furthermore, a synergistic effect on breast cancer (MCF-7) and melanoma (SK-MEL-5) was proven.

PubMed: 33275431
DOI: 10.1021/acs.jmedchem.0c00478

実験手法

X-RAY DIFFRACTION (1.8 Å)