6S92

Crystal structure of group A of Usutu virus envelope protein domain III

6S92 の概要

• エントリーDOI: 10.2210/pdb6s92/pdb
• 分子名称: Genome polyprotein (2 entities in total)
• 機能のキーワード: diii, usutu virus, usuv, envelope protein, viral protein
• 由来する生物種: Usutu virus (USUV)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10992.41

構造登録者

Schoenenwald, A.K.J.,Skern, T. (登録日: 2019-07-11, 公開日: 2020-08-26, 最終更新日: 2024-10-16)

主引用文献

Josephine Schoenenwald, A.K.,Pletzer, M.,Skern, T.
Structural and antigenic investigation of Usutu virus envelope protein domain III.
Virology, 551:46-57, 2020

PubMed Abstract: The mosquito-borne flavivirus Usutu virus (USUV) has recently emerged in birds and humans in Europe. Symptoms of a USUV infection resemble those of West Nile virus (WNV); further, the close antigenic relationship of domain III (DIII) of the USUV and WNV envelope (E) proteins has prevented the development of a reliable serological test to distinguish USUV from WNV. To begin to address this deficiency, we identified ten different sequence groups of DIII from 253 complete and 80 partial USUV genome sequences. We solved the DIII structures of four groups, including that of the outlying CAR-1969 strain, which shows an atypical DIII structure. Structural comparisons of the USUV DIII groups and the DIII of WNV bound to the neutralizing antibody E16 revealed why the E16 failed to neutralize all USUV strains tested except for USUV CAR-1969. The analyses allowed predictions to be made to engineer an antibody specific for USUV CAR-1969.

PubMed: 33011522
DOI: 10.1016/j.virol.2020.09.002

実験手法

X-RAY DIFFRACTION (1.93 Å)