6S8R
D. melanogaster RNA helicase Me31B in complex with GIGYF
Summary for 6S8R
Entry DOI | 10.2210/pdb6s8r/pdb |
Descriptor | ATP-dependent RNA helicase me31b, GIGYF family protein CG11148, ACETATE ION, ... (4 entities in total) |
Functional Keywords | translation, translational control, mrna decay, mirna, rna binding protein |
Biological source | Drosophila melanogaster (Fruit fly) More |
Total number of polymer chains | 2 |
Total formula weight | 23088.45 |
Authors | Peter, D.,Valkov, E. (deposition date: 2019-07-10, release date: 2019-09-04, Last modification date: 2024-10-23) |
Primary citation | Peter, D.,Ruscica, V.,Bawankar, P.,Weber, R.,Helms, S.,Valkov, E.,Igreja, C.,Izaurralde, E. Molecular basis for GIGYF-Me31B complex assembly in 4EHP-mediated translational repression. Genes Dev., 33:1355-1360, 2019 Cited by PubMed Abstract: GIGYF (Grb10-interacting GYF [glycine-tyrosine-phenylalanine domain]) proteins coordinate with 4EHP (eIF4E [eukaryotic initiation factor 4E] homologous protein), the DEAD (Asp-Glu-Ala-Asp)-box helicase Me31B/DDX6, and mRNA-binding proteins to elicit transcript-specific repression. However, the underlying molecular mechanism remains unclear. Here, we report that GIGYF contains a motif necessary and sufficient for direct interaction with Me31B/DDX6. A 2.4 Å crystal structure of the GIGYF-Me31B complex reveals that this motif arranges into a coil connected to a β hairpin on binding to conserved hydrophobic patches on the Me31B RecA2 domain. Structure-guided mutants indicate that 4EHP-GIGYF-DDX6 complex assembly is required for tristetraprolin-mediated down-regulation of an AU-rich mRNA, thus revealing the molecular principles of translational repression. PubMed: 31439631DOI: 10.1101/gad.329219.119 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.41 Å) |
Structure validation
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