6S7L

Arbitrium receptor from a Bacillus subtilis Katmira33 phage

6S7L の概要

• エントリーDOI: 10.2210/pdb6s7l/pdb
• 関連するPDBエントリー: 6S7I
• 分子名称: Arbitrium receptor, GLY-ILE-VAL-ARG-GLY-ALA, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: arbitrium, dna binding protein
• 由来する生物種: Bacillus subtilis; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 91995.26

構造登録者

Marina, A.,Gallego del Sol, F. (登録日: 2019-07-05, 公開日: 2021-04-14, 最終更新日: 2024-01-24)

主引用文献

Gallego Del Sol, F.,Quiles-Puchalt, N.,Brady, A.,Penades, J.R.,Marina, A.
Insights into the mechanism of action of the arbitrium communication system in SPbeta phages.
Nat Commun, 13:3627-3627, 2022

PubMed Abstract: The arbitrium system is employed by phages of the SPbeta family to communicate with their progeny during infection to decide either to follow the lytic or the lysogenic cycle. The system is controlled by a peptide, AimP, that binds to the regulator AimR, inhibiting its DNA-binding activity and expression of aimX. Although the structure of AimR has been elucidated for phages SPβ and phi3T, there is still controversy regarding the molecular mechanism of AimR function, with two different proposed models for SPβ. In this study, we deepen our understanding of the system by solving the structure of an additional AimR that shows chimerical characteristics with the SPβ receptor. The crystal structures of this AimR (apo, AimP-bound and DNA-bound) together with in vitro and in vivo analyses confirm a mechanism of action by AimP-induced conformational restriction, shedding light on peptide specificity and cross regulation with relevant biological implications.

PubMed: 35750663
DOI: 10.1038/s41467-022-31144-3

実験手法

X-RAY DIFFRACTION (2.6 Å)