6S6S

Structure of Azospirillum brasilense Glutamate Synthase in a4b4 oligomeric state.

6S6S の概要

• エントリーDOI: 10.2210/pdb6s6s/pdb
• EMDBエントリー: 10104
• 分子名称: Glutamate synthase [NADPH] large chain, Glutamate synthase [NADPH] small chain, FLAVIN MONONUCLEOTIDE, ... (6 entities in total)
• 機能のキーワード: glutamate synthesys, complex, oligomeric assemblies, flavoprotein
• 由来する生物種: Azospirillum brasilense; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 883563.25

構造登録者

Chaves-Sanjuan, A.,Bolognesi, M. (登録日: 2019-07-03, 公開日: 2019-09-11, 最終更新日: 2025-10-01)

主引用文献

Swuec, P.,Chaves-Sanjuan, A.,Camilloni, C.,Vanoni, M.A.,Bolognesi, M.
Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in Its Oligomeric Assemblies.
J.Mol.Biol., 431:4523-4526, 2019

PubMed Abstract: Bacterial NADPH-dependent glutamate synthase (GltS) is a complex iron-sulfur flavoprotein that catalyzes the reductive synthesis of two L-Glu molecules from L-Gln and 2-oxo-glutarate. GltS functional unit hosts an α-subunit (αGltS) and a β-subunit (βGltS) that assemble in different αβ oligomers in solution. Here, we present the cryo-electron microscopy structures of Azospirillum brasilense GltS in four different oligomeric states (αβ, αβ, αβ and αβ, in the 3.5- to 4.1-Å resolution range). Our study provides a comprehensive GltS model that details the inter-protomeric assemblies and allows unequivocal location of the FAD cofactor and of two electron transfer [4Fe-4S] clusters within βGltS.

PubMed: 31473159
DOI: 10.1016/j.jmb.2019.08.011

実験手法

ELECTRON MICROSCOPY (3.9 Å)