6S67

Structure of the Fluorescent Protein AausFP1 from Aequorea cf. australis at pH 7.0

6S67 の概要

• エントリーDOI: 10.2210/pdb6s67/pdb
• 分子名称: Aequorea cf. australis fluorescent protein 1 (AausFP1), GLYCEROL (3 entities in total)
• 機能のキーワード: gfp-like fluorescent protein, fluorescent protein
• 由来する生物種: Aequorea australis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 103401.38

構造登録者

Depernet, H.,Gotthard, G.,Lambert, G.G.,Shaner, N.C.,Royant, A. (登録日: 2019-07-02, 公開日: 2020-07-22, 最終更新日: 2024-11-06)

主引用文献

Lambert, G.G.,Depernet, H.,Gotthard, G.,Schultz, D.T.,Navizet, I.,Lambert, T.,Adams, S.R.,Torreblanca-Zanca, A.,Chu, M.,Bindels, D.S.,Levesque, V.,Nero Moffatt, J.,Salih, A.,Royant, A.,Shaner, N.C.
Aequorea's secrets revealed: New fluorescent proteins with unique properties for bioimaging and biosensing.
Plos Biol., 18:e3000936-e3000936, 2020

PubMed Abstract: Using mRNA sequencing and de novo transcriptome assembly, we identified, cloned, and characterized 9 previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related Aequorea species, with most sequences highly divergent from A. victoria green fluorescent protein (avGFP). Among these FPs are the brightest green fluorescent protein (GFP) homolog yet characterized and a reversibly photochromic FP that responds to UV and blue light. Beyond green emitters, Aequorea species express purple- and blue-pigmented chromoproteins (CPs) with absorbances ranging from green to far-red, including 2 that are photoconvertible. X-ray crystallography revealed that Aequorea CPs contain a chemically novel chromophore with an unexpected crosslink to the main polypeptide chain. Because of the unique attributes of several of these newly discovered FPs, we expect that Aequorea will, once again, give rise to an entirely new generation of useful probes for bioimaging and biosensing.

PubMed: 33137097
DOI: 10.1371/journal.pbio.3000936

実験手法

X-RAY DIFFRACTION (2.47 Å)