6S2P

Structure of the NB-ARC domain from the Tomato immune receptor NRC1

6S2P の概要

• エントリーDOI: 10.2210/pdb6s2p/pdb
• 分子名称: NRC1, ADENOSINE-5'-DIPHOSPHATE (2 entities in total)
• 機能のキーワード: nlr receptor, nb-arc domain, plant immunity, plant protein
• 由来する生物種: Solanum lycopersicum (Tomato)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40578.38

構造登録者

Steele, J.F.C.,Banfield, M.J. (登録日: 2019-06-21, 公開日: 2019-07-03, 最終更新日: 2024-05-15)

主引用文献

Steele, J.F.C.,Hughes, R.K.,Banfield, M.J.
Structural and biochemical studies of an NB-ARC domain from a plant NLR immune receptor.
Plos One, 14:e0221226-e0221226, 2019

PubMed Abstract: Plant NLRs are modular immune receptors that trigger rapid cell death in response to attempted infection by pathogens. A highly conserved nucleotide-binding domain shared with APAF-1, various R-proteins and CED-4 (NB-ARC domain) is proposed to act as a molecular switch, cycling between ADP (repressed) and ATP (active) bound forms. Studies of plant NLR NB-ARC domains have revealed functional similarities to mammalian homologues, and provided insight into potential mechanisms of regulation. However, further advances have been limited by difficulties in obtaining sufficient yields of protein suitable for structural and biochemical techniques. From protein expression screens in Escherichia coli and Sf9 insect cells, we defined suitable conditions to produce the NB-ARC domain from the tomato NLR NRC1. Biophysical analyses of this domain showed it is a folded, soluble protein. Structural studies revealed the NRC1 NB-ARC domain had co-purified with ADP, and confirmed predicted structural similarities between plant NLR NB-ARC domains and their mammalian homologues.

PubMed: 31461469
DOI: 10.1371/journal.pone.0221226

実験手法

X-RAY DIFFRACTION (2.5 Å)