6S2C

Acquired functional capsid structures in metazoan totivirus-like dsRNA virus.

6S2C の概要

• エントリーDOI: 10.2210/pdb6s2c/pdb
• EMDBエントリー: 10087
• 分子名称: Capsid protein (2 entities in total)
• 機能のキーワード: mosquito, totivirus, totiviridae, totivirus-like virus, dsrna, capsid, virus
• 由来する生物種: Omono River virus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 182639.69

構造登録者

Okamoto, K.,Larsson, S.D.D.,Maia, R.N.C.F.,Murata, K.,Hajdu, J.,Iwasaki, K.,Miyazaki, N. (登録日: 2019-06-20, 公開日: 2020-04-29, 最終更新日: 2024-05-22)

主引用文献

Okamoto, K.,Ferreira, R.J.,Larsson, D.S.D.,Maia, F.R.N.C.,Isawa, H.,Sawabe, K.,Murata, K.,Hajdu, J.,Iwasaki, K.,Kasson, P.M.,Miyazaki, N.
Acquired Functional Capsid Structures in Metazoan Totivirus-like dsRNA Virus.
Structure, 28:888-896.e3, 2020

PubMed Abstract: Non-enveloped icosahedral double-stranded RNA (dsRNA) viruses possess multifunctional capsids required for their proliferation. Whereas protozoan/fungal dsRNA viruses have a relatively simple capsid structure, which suffices for the intracellular phase in their life cycle, metazoan dsRNA viruses have acquired additional structural features as an adaptation for extracellular cell-to-cell transmission in multicellular hosts. Here, we present the first atomic model of a metazoan dsRNA totivirus-like virus and the structure reveals three unique structural traits: a C-terminal interlocking arm, surface projecting loops, and an obstruction at the pore on the 5-fold symmetry axis. These traits are keys to understanding the capsid functions of metazoan dsRNA viruses, such as particle stability and formation, cell entry, and endogenous intraparticle transcription of mRNA. On the basis of molecular dynamics simulations of the obstructed pore, we propose a possible mechanism of intraparticle transcription in totivirus-like viruses, which dynamically switches between open and closed states of the pore(s).

PubMed: 32413288
DOI: 10.1016/j.str.2020.04.016

実験手法

ELECTRON MICROSCOPY (3.2 Å)