6RXI

In-flow serial synchrotron crystallography using a 3D-printed microfluidic device (3D-MiXD): Lysozyme

6RXI の概要

• エントリーDOI: 10.2210/pdb6rxi/pdb
• 分子名称: Lysozyme C, DI(HYDROXYETHYL)ETHER, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14601.84

構造登録者

Monteiro, D.C.F.,von Stetten, D.,Pearson, A.R.,Trebbin, M. (登録日: 2019-06-08, 公開日: 2020-03-18, 最終更新日: 2024-10-16)

主引用文献

Monteiro, D.C.F.,von Stetten, D.,Stohrer, C.,Sans, M.,Pearson, A.R.,Santoni, G.,van der Linden, P.,Trebbin, M.
3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow.
Iucrj, 7:207-219, 2020

PubMed Abstract: Serial crystallography has enabled the study of complex biological questions through the determination of biomolecular structures at room temperature using low X-ray doses. Furthermore, it has enabled the study of protein dynamics by the capture of atomically resolved and time-resolved molecular movies. However, the study of many biologically relevant targets is still severely hindered by high sample consumption and lengthy data-collection times. By combining serial synchrotron crystallography (SSX) with 3D printing, a new experimental platform has been created that tackles these challenges. An affordable 3D-printed, X-ray-compatible microfluidic device (3D-MiXD) is reported that allows data to be collected from protein microcrystals in a 3D flow with very high hit and indexing rates, while keeping the sample consumption low. The miniaturized 3D-MiXD can be rapidly installed into virtually any synchrotron beamline with only minimal adjustments. This efficient collection scheme in combination with its mixing geometry paves the way for recording molecular movies at synchrotrons by mixing-triggered millisecond time-resolved SSX.

PubMed: 32148849
DOI: 10.1107/S2052252519016865

実験手法

X-RAY DIFFRACTION (2 Å)