6RWB

Cryo-EM structure of Yersinia pseudotuberculosis TcaA-TcaB

6RWB の概要

• エントリーDOI: 10.2210/pdb6rwb/pdb
• 関連するPDBエントリー: 6RW6 6RW8 6RW9 6RWA
• EMDBエントリー: 10037
• 分子名称: Toxin,Toxin complex subunit TcaB,Putative toxin subunit,Putative toxin subunit,Toxin,Toxin complex subunit TcaB,Putative toxin subunit,Putative toxin subunit,Toxin,Toxin complex subunit TcaB,Putative toxin subunit,Putative toxin subunit,Toxin,Toxin complex subunit TcaB,Putative toxin subunit,Putative toxin subunit (1 entity in total)
• 機能のキーワード: toxin, membrane permeation, translocation, complex
• 由来する生物種: Yersinia pseudotuberculosis; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 1159925.16

構造登録者

Roderer, D.,Leidreiter, F.,Gatsogiannis, C.,Meusch, D.,Benz, R.,Raunser, S. (登録日: 2019-06-04, 公開日: 2019-10-23, 最終更新日: 2024-05-22)

主引用文献

Leidreiter, F.,Roderer, D.,Meusch, D.,Gatsogiannis, C.,Benz, R.,Raunser, S.
Common architecture of Tc toxins from human and insect pathogenic bacteria.
Sci Adv, 5:eaax6497-eaax6497, 2019

PubMed Abstract: Tc toxins use a syringe-like mechanism to penetrate the membrane and translocate toxic enzymes into the host cytosol. They are composed of three components: TcA, TcB, and TcC. Low-resolution structures of TcAs from different bacteria suggest a considerable difference in their architecture and possibly in their mechanism of action. Here, we present high-resolution structures of five TcAs from insect and human pathogens, which show a similar overall composition and domain organization. Essential structural features, including a trefoil protein knot, are present in all TcAs, suggesting a common mechanism of action. All TcAs form functional pores and can be combined with TcB-TcC subunits from other species to form active chimeric holotoxins. We identified a conserved ionic pair that stabilizes the shell, likely operating as a strong latch that only springs open after destabilization of other regions. Our results provide new insights into the architecture and mechanism of the Tc toxin family.

PubMed: 31663026
DOI: 10.1126/sciadv.aax6497

実験手法

ELECTRON MICROSCOPY (3.25 Å)