6RW4

Structure of human mitochondrial 28S ribosome in complex with mitochondrial IF3

これはPDB形式変換不可エントリーです。

6RW4 の概要

• エントリーDOI: 10.2210/pdb6rw4/pdb
• EMDBエントリー: 10021
• 分子名称: 12S mitochondrial rRNA, 28S ribosomal protein S12, mitochondrial, 28S ribosomal protein S14, mitochondrial, ... (42 entities in total)
• 機能のキーワード: ribosomal small subunit, initiation complex, initiation factor 3, mitochondria, ribosome
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 1179569.93

構造登録者

Itoh, Y.,Khawaja, A.,Rorbach, J.,Amunts, A. (登録日: 2019-06-03, 公開日: 2020-06-03, 最終更新日: 2024-04-24)

主引用文献

Khawaja, A.,Itoh, Y.,Remes, C.,Spahr, H.,Yukhnovets, O.,Hofig, H.,Amunts, A.,Rorbach, J.
Distinct pre-initiation steps in human mitochondrial translation.
Nat Commun, 11:2932-2932, 2020

PubMed Abstract: Translation initiation in human mitochondria relies upon specialized mitoribosomes and initiation factors, mtIF2 and mtIF3, which have diverged from their bacterial counterparts. Here we report two distinct mitochondrial pre-initiation assembly steps involving those factors. Single-particle cryo-EM revealed that in the first step, interactions between mitochondria-specific protein mS37 and mtIF3 keep the small mitoribosomal subunit in a conformation favorable for a subsequent accommodation of mtIF2 in the second step. Combination with fluorescence cross-correlation spectroscopy analyses suggests that mtIF3 promotes complex assembly without mRNA or initiator tRNA binding, where exclusion is achieved by the N-terminal and C-terminal domains of mtIF3. Finally, the association of large mitoribosomal subunit is required for initiator tRNA and leaderless mRNA recruitment to form a stable initiation complex. These data reveal fundamental aspects of mammalian protein synthesis that are specific to mitochondria.

PubMed: 32522994
DOI: 10.1038/s41467-020-16503-2

実験手法

ELECTRON MICROSCOPY (2.97 Å)