6RRL

Solution NMR structure of the peptide 3967 from medicinal leech Hirudo medicinalis in dodecylphosphocholine micelles

6RRL の概要

• エントリーDOI: 10.2210/pdb6rrl/pdb
• NMR情報: BMRB: 34401
• 分子名称: peptide 3967 (1 entity in total)
• 機能のキーワード: antimicrobial peptides, medicinal leech, hirudo medicinalis, genome, prediction algorithm, dooecylphosphocholine micelles, antimicrobial protein
• 由来する生物種: Hirudo medicinalis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1562.07

構造登録者

Nadezhdin, K.D.,Grafskaia, E.N.,Arseniev, A.S.,Lazarev, V.N. (登録日: 2019-05-20, 公開日: 2019-07-24, 最終更新日: 2024-06-19)

主引用文献

Grafskaia, E.N.,Nadezhdin, K.D.,Talyzina, I.A.,Polina, N.F.,Podgorny, O.V.,Pavlova, E.R.,Bashkirov, P.V.,Kharlampieva, D.D.,Bobrovsky, P.A.,Latsis, I.A.,Manuvera, V.A.,Babenko, V.V.,Trukhan, V.M.,Arseniev, A.S.,Klinov, D.V.,Lazarev, V.N.
Medicinal leech antimicrobial peptides lacking toxicity represent a promising alternative strategy to combat antibiotic-resistant pathogens.
Eur.J.Med.Chem., 180:143-153, 2019

PubMed Abstract: The rise of antibiotic resistance has necessitated the development of alternative strategies for the treatment of infectious diseases. Antimicrobial peptides (AMPs), components of the innate immune response in various organisms, are promising next-generation drugs against bacterial infections. The ability of the medicinal leech Hirudo medicinalis to store blood for months with little change has attracted interest regarding the identification of novel AMPs in this organism. In this study, we employed computational algorithms to the medicinal leech genome assembly to identify amino acid sequences encoding potential AMPs. Then, we synthesized twelve candidate AMPs identified by the algorithms, determined their secondary structures, measured minimal inhibitory concentrations against three bacterial species (Escherichia coli, Bacillus subtilis, and Chlamydia thrachomatis), and assayed cytotoxic and haemolytic activities. Eight of twelve candidate AMPs possessed antimicrobial activity, and only two of them, 3967 (FRIMRILRVLKL) and 536-1 (RWRLVCFLCRRKKV), exhibited inhibition of growth of all tested bacterial species at a minimal inhibitory concentration of 10 μmol. Thus, we evidence the utility of the developed computational algorithms for the identification of AMPs with low toxicity and haemolytic activity in the medicinal leech genome assembly.

PubMed: 31302447
DOI: 10.1016/j.ejmech.2019.06.080

実験手法

SOLUTION NMR