6RND

Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Xylose Isomerase 15 ms timepoint

6RND の概要

• エントリーDOI: 10.2210/pdb6rnd/pdb
• 分子名称: Xylose isomerase, MAGNESIUM ION, alpha-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: isomerase, complex, diffusion, dynamics
• 由来する生物種: Streptomyces rubiginosus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43512.06

構造登録者

Mehrabi, P.,Schulz, E.C.,Miller, R.J.D. (登録日: 2019-05-08, 公開日: 2019-10-02, 最終更新日: 2024-01-24)

主引用文献

Mehrabi, P.,Schulz, E.C.,Agthe, M.,Horrell, S.,Bourenkov, G.,von Stetten, D.,Leimkohl, J.P.,Schikora, H.,Schneider, T.R.,Pearson, A.R.,Tellkamp, F.,Miller, R.J.D.
Liquid application method for time-resolved analyses by serial synchrotron crystallography.
Nat.Methods, 16:979-982, 2019

PubMed Abstract: We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.

PubMed: 31527838
DOI: 10.1038/s41592-019-0553-1

実験手法

X-RAY DIFFRACTION (1.7 Å)