6RLX

X-RAY STRUCTURE OF HUMAN RELAXIN AT 1.5 ANGSTROMS. COMPARISON TO INSULIN AND IMPLICATIONS FOR RECEPTOR BINDING DETERMINANTS

6RLX の概要

• エントリーDOI: 10.2210/pdb6rlx/pdb
• 分子名称: RELAXIN, A-CHAIN, RELAXIN, B-CHAIN (3 entities in total)
• 機能のキーワード: hormone(muscle relaxant)
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 11723.95

構造登録者

Eigenbrot, C.,Randal, M.,Kossiakoff, A.A. (登録日: 1991-06-21, 公開日: 1993-10-31, 最終更新日: 2024-10-09)

主引用文献

Eigenbrot, C.,Randal, M.,Quan, C.,Burnier, J.,O'Connell, L.,Rinderknecht, E.,Kossiakoff, A.A.
X-ray structure of human relaxin at 1.5 A. Comparison to insulin and implications for receptor binding determinants.
J.Mol.Biol., 221:15-21, 1991

PubMed Abstract: The X-ray crystal structure of relaxin at 1.5 A resolution is reported for the physiologically active form of the human hormone. Relaxin is a small, two-chain polypeptide that is a member of the protein hormone family that also includes insulin and the insulin-like growth factors IGF-I and IGF-II. These hormones have biologically diverse activities but are structurally similar, sharing a distinctive pattern of cysteine and glycine residues. The predicted structural homology of relaxin to insulin is confirmed by this structural analysis; however, there are significant differences in the terminal regions of the b-chain. Although relaxin, like insulin, crystallizes as a dimer, the orientation of the molecules in the respective dimers is completely different. The region of the relaxin molecule proposed to be involved in receptor binding is part of the dimer interface, suggesting that some of the other residues contained in the dimer contact surface might be receptor binding determinants as well. The proposed receptor binding determinants for insulin likewise include residues at its dimer interface. However, because the dimer contacts of relaxin and insulin are quite different, it appears that these two structurally related hormones have evolved somewhat dissimilar mechanisms for receptor binding.

PubMed: 1656049

実験手法

X-RAY DIFFRACTION (1.5 Å)