6RK2

Complex structure of virulence factor SghA mutant with its substrate SAG

Summary for 6RK2

• Entry DOI: 10.2210/pdb6rk2/pdb
• Descriptor: Beta-glucosidase, 2-(alpha-L-altropyranosyloxy)benzoic acid (3 entities in total)
• Functional Keywords: chemical signaling, sucrose, agrobacterium, host-pathogen interaction, glucosidase, hydrolase
• Biological source: Agrobacterium tumefaciens A6
• Total number of polymer chains: 2
• Total formula weight: 110263.62

Authors

Ye, F.Z.,Wang, C.,Chang, C.Q.,Zhang, L.H.,Gao, Y.G. (deposition date: 2019-04-30, release date: 2019-10-09, Last modification date: 2024-01-24)

Primary citation

Wang, C.,Ye, F.,Chang, C.,Liu, X.,Wang, J.,Wang, J.,Yan, X.F.,Fu, Q.,Zhou, J.,Chen, S.,Gao, Y.G.,Zhang, L.H.
Agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals.
Proc.Natl.Acad.Sci.USA, 116:22331-22340, 2019

PubMed Abstract: It is highly intriguing how bacterial pathogens can quickly shut down energy-costly infection machinery once successful infection is established. This study depicts that mutation of repressor SghR increases the expression of hydrolase SghA in , which releases plant defense signal salicylic acid (SA) from its storage form SA β-glucoside (SAG). Addition of SA substantially reduces gene expression of bacterial virulence. Bacterial genes and are differentially transcribed at early and later infection stages, respectively. Plant metabolite sucrose is a signal ligand that inactivates SghR and consequently induces expression. Disruption of leads to increased expression and enhances tumor formation whereas mutation of decreases expression and tumor formation. These results depict a remarkable mechanism by which taps on the reserved pool of plant signal SA to reprogram its virulence upon establishment of infection.

PubMed: 31604827
DOI: 10.1073/pnas.1903695116

Experimental method

X-RAY DIFFRACTION (2.09 Å)