6RJX

Crystal structure of the N-terminal domain of Lyme disease agent Borrelia burgdorferi major virulence factor BB0323 (native data)

6RJX の概要

• エントリーDOI: 10.2210/pdb6rjx/pdb
• 分子名称: LysM domain protein (1 entity in total)
• 機能のキーワード: lipoprotein, spectrin repeats, structural protein
• 由来する生物種: Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22249.20

構造登録者

Brangulis, K.,Akopjana, I.,Kazaks, A.,Tars, K. (登録日: 2019-04-29, 公開日: 2019-09-11, 最終更新日: 2024-01-24)

主引用文献

Brangulis, K.,Akopjana, I.,Kazaks, A.,Tars, K.
Crystal structure of the N-terminal domain of the major virulence factor BB0323 from the Lyme disease agent Borrelia burgdorferi.
Acta Crystallogr D Struct Biol, 75:825-830, 2019

PubMed Abstract: Lyme disease is an infection caused by the spirochete Borrelia burgdorferi after it is transmitted to a mammalian organism during a tick blood meal. B. burgdorferi encodes at least 140 lipoproteins located on the outer or inner membrane, thus facing the surroundings or the periplasmic space, respectively. However, most of the predicted lipoproteins are of unknown function, and only a few proteins are known to be essential for the persistence and virulence of the pathogen. One such protein is the periplasmic BB0323, which is indispensable for B. burgdorferi to cause Lyme disease and the function of which is associated with cell fission and outer membrane integrity. After expression and transport to the periplasm, BB0323 is cleaved into C-terminal and N-terminal domains by the periplasmic serine protease BB0104. The resulting N-terminal domain is sufficient to ensure the survival of B. burgdorferi throughout the mouse-tick infection cycle. The crystal structure of the N-terminal domain of BB0323 was determined at 2.35 Å resolution. The overall fold of the protein belongs to the spectrin superfamily, with the characteristic interconnected triple-helical bundles known as spectrin repeats that function as linkers between different cell components in other organisms. Overall, the reported three-dimensional structure of the N-terminal domain of BB0323 not only reveals the molecular details of a protein that is essential for B. burgdorferi membrane integrity, cell fission and infectivity, but also suggests that spectrin repeats in bacteria are not limited to the EzrA proteins.

PubMed: 31478905
DOI: 10.1107/S2059798319010751

実験手法

X-RAY DIFFRACTION (2.35 Å)