6RJM

Complex structure of virulence factor SghA and its hydrolysis product glucose

6RJM の概要

• エントリーDOI: 10.2210/pdb6rjm/pdb
• 分子名称: Beta-glucosidase, alpha-D-glucopyranose (3 entities in total)
• 機能のキーワード: chemical signaling, sucrose, agrobacterium, host-pathogen interaction, glucosidase, hydrolase
• 由来する生物種: Agrobacterium tumefaciens A6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 110107.48

構造登録者

Ye, F.Z.,Wang, C.,Chang, C.Q.,Zhang, L.H.,Gao, Y.G. (登録日: 2019-04-27, 公開日: 2019-10-09, 最終更新日: 2024-01-24)

主引用文献

Wang, C.,Ye, F.,Chang, C.,Liu, X.,Wang, J.,Wang, J.,Yan, X.F.,Fu, Q.,Zhou, J.,Chen, S.,Gao, Y.G.,Zhang, L.H.
Agrobacteria reprogram virulence gene expression by controlled release of host-conjugated signals.
Proc.Natl.Acad.Sci.USA, 116:22331-22340, 2019

PubMed Abstract: It is highly intriguing how bacterial pathogens can quickly shut down energy-costly infection machinery once successful infection is established. This study depicts that mutation of repressor SghR increases the expression of hydrolase SghA in , which releases plant defense signal salicylic acid (SA) from its storage form SA β-glucoside (SAG). Addition of SA substantially reduces gene expression of bacterial virulence. Bacterial genes and are differentially transcribed at early and later infection stages, respectively. Plant metabolite sucrose is a signal ligand that inactivates SghR and consequently induces expression. Disruption of leads to increased expression and enhances tumor formation whereas mutation of decreases expression and tumor formation. These results depict a remarkable mechanism by which taps on the reserved pool of plant signal SA to reprogram its virulence upon establishment of infection.

PubMed: 31604827
DOI: 10.1073/pnas.1903695116

実験手法

X-RAY DIFFRACTION (2.112 Å)