6RJ0

Atlantic Cod Nervous Necrosis Virus-Like Particle produced in Nicotiana benthamiana

Summary for 6RJ0

• Entry DOI: 10.2210/pdb6rj0/pdb
• EMDB information: 4899
• Descriptor: Coat protein (1 entity in total)
• Functional Keywords: betanodavirus, virus-like particle, virus, vaccine, virus like particle
• Biological source: Betanodavirus sp.
• Total number of polymer chains: 3
• Total formula weight: 110711.13

Authors

Hurdiss, D.L.,Ranson, N.A. (deposition date: 2019-04-25, release date: 2019-07-17, Last modification date: 2024-05-22)

Primary citation

Marsian, J.,Hurdiss, D.L.,Ranson, N.A.,Ritala, A.,Paley, R.,Cano, I.,Lomonossoff, G.P.
Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease.
Front Plant Sci, 10:880-880, 2019

PubMed Abstract: Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass () showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

PubMed: 31354759
DOI: 10.3389/fpls.2019.00880

Experimental method

ELECTRON MICROSCOPY (3.7 Å)