6RJ0

Atlantic Cod Nervous Necrosis Virus-Like Particle produced in Nicotiana benthamiana

6RJ0 の概要

• エントリーDOI: 10.2210/pdb6rj0/pdb
• EMDBエントリー: 4899
• 分子名称: Coat protein (1 entity in total)
• 機能のキーワード: betanodavirus, virus-like particle, virus, vaccine, virus like particle
• 由来する生物種: Betanodavirus sp.
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 110711.13

構造登録者

Hurdiss, D.L.,Ranson, N.A. (登録日: 2019-04-25, 公開日: 2019-07-17, 最終更新日: 2024-05-22)

主引用文献

Marsian, J.,Hurdiss, D.L.,Ranson, N.A.,Ritala, A.,Paley, R.,Cano, I.,Lomonossoff, G.P.
Plant-Made Nervous Necrosis Virus-Like Particles Protect Fish Against Disease.
Front Plant Sci, 10:880-880, 2019

PubMed Abstract: Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass () showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

PubMed: 31354759
DOI: 10.3389/fpls.2019.00880

実験手法

ELECTRON MICROSCOPY (3.7 Å)