6RIW

PfaC Keto synthase-Chain length factor

6RIW の概要

• エントリーDOI: 10.2210/pdb6riw/pdb
• 分子名称: ORF10, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: pufa synthase, pks synthase, ketosynthase, chain length factor
• 由来する生物種: Moritella marina
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 108352.04

構造登録者

Santin, O.,Moncalian, G. (登録日: 2019-04-25, 公開日: 2020-08-26, 最終更新日: 2024-01-24)

主引用文献

Santin, O.,Yuet, K.,Khosla, C.,Moncalian, G.
Structure and Mechanism of the Ketosynthase-Chain Length Factor Didomain from a Prototypical Polyunsaturated Fatty Acid Synthase.
Biochemistry, 59:4735-4743, 2020

PubMed Abstract: Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential ingredients of the human diet. They are synthesized by LC-PUFA synthases (PFASs) expressed in marine bacteria and other organisms. PFASs are large enzyme complexes that are homologous to mammalian fatty acid synthases and microbial polyketide synthases. One subunit of each PFAS harbors consecutive ketosynthase (KSc) and chain length factor (CLF) domains that collectively catalyze the elongation of a nascent fatty acyl chain via iterative carbon-carbon bond formation. We report the X-ray crystal structure of the KS-CLF didomain from a well-studied PFAS in . Our structure, in combination with biochemical analysis, provides a foundation for understanding the mechanism of substrate recognition and chain length control by the KS-CLF didomain as well as its interaction with a cognate acyl carrier protein partner.

PubMed: 33283513
DOI: 10.1021/acs.biochem.0c00785

実験手法

X-RAY DIFFRACTION (1.85 Å)