6RIQ
MinCD filament from Pseudomonas aeruginosa
Summary for 6RIQ
Entry DOI | 10.2210/pdb6riq/pdb |
EMDB information | 4897 |
Descriptor | MinC, Site-determining protein, MAGNESIUM ION, ... (4 entities in total) |
Functional Keywords | bacterial cell division, protein fibril |
Biological source | Pseudomonas aeruginosa More |
Total number of polymer chains | 22 |
Total formula weight | 498782.92 |
Authors | Szewczak-Harris, A.,Wagstaff, J.,Lowe, J. (deposition date: 2019-04-24, release date: 2019-06-19, Last modification date: 2025-04-09) |
Primary citation | Szewczak-Harris, A.,Wagstaff, J.,Lowe, J. Cryo-EM structure of the MinCD copolymeric filament from Pseudomonas aeruginosa at 3.1 angstrom resolution. Febs Lett., 593:1915-1926, 2019 Cited by PubMed Abstract: Positioning of the division site in many bacterial species relies on the MinCDE system, which prevents the cytokinetic Z-ring from assembling anywhere but the mid-cell, through an oscillatory diffusion-reaction mechanism. MinD dimers bind to membranes and, via their partner MinC, inhibit the polymerization of cell division protein FtsZ into the Z-ring. MinC and MinD form polymeric assemblies in solution and on cell membranes. Here, we report the high-resolution cryo-EM structure of the copolymeric filaments of Pseudomonas aeruginosa MinCD. The filaments consist of three protofilaments made of alternating MinC and MinD dimers. The MinCD protofilaments are almost completely straight and assemble as single protofilaments on lipid membranes, which we also visualized by cryo-EM. PubMed: 31166018DOI: 10.1002/1873-3468.13471 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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