6RHU

Single crystal serial study of the inhibition of laccases from Steccherinum murashkinskyi by chloride anions at sub-atomic resolution. Second structure of the series with 165 KGy dose.

6RHU の概要

• エントリーDOI: 10.2210/pdb6rhu/pdb
• 分子名称: Laccase 2, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, COPPER (II) ION, ... (6 entities in total)
• 機能のキーワード: multi copper oxidase, laccase, complex with molecular oxygen, oxidoreductase
• 由来する生物種: Steccherinum murashkinskyi
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 54481.08

構造登録者

Polyakov, K.M.,Gavryushov, S.,Fedorova, T.V.,Glazunova, O.A.,Popov, A.N. (登録日: 2019-04-22, 公開日: 2019-05-01, 最終更新日: 2024-01-24)

主引用文献

Polyakov, K.M.,Gavryushov, S.,Fedorova, T.V.,Glazunova, O.A.,Popov, A.N.
The subatomic resolution study of laccase inhibition by chloride and fluoride anions using single-crystal serial crystallography: insights into the enzymatic reaction mechanism.
Acta Crystallogr D Struct Biol, 75:804-816, 2019

PubMed Abstract: Laccases are enzymes that catalyze the oxidation of a wide range of organic and inorganic substrates accompanied by the reduction of molecular oxygen to water. Here, a subatomic resolution X-ray crystallographic study of the mechanism of inhibition of the laccase from the basidiomycete fungus Steccherinum murashkinskyi by chloride and fluoride ions is presented. Three series of X-ray diffraction data sets were collected with increasing doses of absorbed X-ray radiation from a native S. murashkinskyi laccase crystal and from crystals of complexes of the laccase with chloride and fluoride ions. The data for the native laccase crystal confirmed the previously deduced enzymatic mechanism of molecular oxygen reduction. The structures of the complexes allowed the localization of chloride and fluoride ions in the channel near the T2 copper ion. These ions replace the oxygen ligand of the T2 copper ion in this channel and can play the role of this ligand in the enzymatic reaction. As follows from analysis of the structures from the increasing dose series, the inhibition of laccases by chloride and fluoride anions can be explained by the fact that the binding of these negatively charged ions at the position of the oxygen ligand of the T2 copper ion impedes the reduction of the T2 copper ion.

PubMed: 31478903
DOI: 10.1107/S2059798319010684

実験手法

X-RAY DIFFRACTION (0.96 Å)