6REK

Crystal structure of Pizza6-SH with Cu2+

6REK の概要

• エントリーDOI: 10.2210/pdb6rek/pdb
• 分子名称: Pizza6-SH, COPPER (II) ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: artificial beta-propeller protein, de novo protein, computational design, copper, pizza, metalloprotein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26155.23

構造登録者

Noguchi, H.,Clarke, D.E.,Gryspeerdt, J.L.,Feyter, S.D.,Voet, A.R.D. (登録日: 2019-04-12, 公開日: 2019-07-31, 最終更新日: 2024-01-24)

主引用文献

Clarke, D.E.,Noguchi, H.,Gryspeerdt, J.A.G.,De Feyter, S.,Voet, A.R.D.
Artificial beta-propeller protein-based hydrolases.
Chem.Commun.(Camb.), 55:8880-8883, 2019

PubMed Abstract: We developed an artificial hydrolase based on the symmetrical Pizza6 β-propeller protein for the metal-free hydrolysis of 4-nitrophenyl acetate and butyrate. Through site-specific mutagenesis and crystallisation studies, the catalytic mechanism was investigated and found to be dependent on a threonine-histidine dyad. The mutant with additional histidine residues generated the highest kcat values, forming a His-His-Thr triad and matched previously reported metalloenzymes. The highly symmetrical β-propeller artificial enzymes and their protein-metal complexes have potential to be utilised in bioinorganic and supramolecular chemistry, as well as being developed further into 2D/3D catalytic materials.

PubMed: 31321399
DOI: 10.1039/c9cc04388h

実験手法

X-RAY DIFFRACTION (1.1 Å)