6REG
Crystal structure of Pizza6-S with Zn2+
Summary for 6REG
| Entry DOI | 10.2210/pdb6reg/pdb |
| Descriptor | Pizza6-S, ZINC ION, GLYCEROL, ... (4 entities in total) |
| Functional Keywords | artificial beta-propeller protein, de novo protein, computational design, zinc, pizza, metalloprotein |
| Biological source | synthetic construct |
| Total number of polymer chains | 2 |
| Total formula weight | 52315.59 |
| Authors | Noguchi, H.,Clarke, D.E.,Gryspeerdt, J.L.,Feyter, S.D.,Voet, A.R.D. (deposition date: 2019-04-12, release date: 2019-07-31, Last modification date: 2024-01-24) |
| Primary citation | Clarke, D.E.,Noguchi, H.,Gryspeerdt, J.A.G.,De Feyter, S.,Voet, A.R.D. Artificial beta-propeller protein-based hydrolases. Chem.Commun.(Camb.), 55:8880-8883, 2019 Cited by PubMed Abstract: We developed an artificial hydrolase based on the symmetrical Pizza6 β-propeller protein for the metal-free hydrolysis of 4-nitrophenyl acetate and butyrate. Through site-specific mutagenesis and crystallisation studies, the catalytic mechanism was investigated and found to be dependent on a threonine-histidine dyad. The mutant with additional histidine residues generated the highest kcat values, forming a His-His-Thr triad and matched previously reported metalloenzymes. The highly symmetrical β-propeller artificial enzymes and their protein-metal complexes have potential to be utilised in bioinorganic and supramolecular chemistry, as well as being developed further into 2D/3D catalytic materials. PubMed: 31321399DOI: 10.1039/c9cc04388h PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.3 Å) |
Structure validation
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