6RA6

Ferric murine neuroglobin Gly-loop44-47/F106A mutant

6RA6 の概要

• エントリーDOI: 10.2210/pdb6ra6/pdb
• 分子名称: Neuroglobin,Murine neuroglobin mutant,Neuroglobin, PROTOPORPHYRIN IX CONTAINING FE, SULFATE ION, ... (7 entities in total)
• 機能のキーワード: heme protein, globin, gas binding, oxygen binding
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17801.96

構造登録者

Exertier, C.,Freda, I.,Montemiglio, L.C.,Savino, C.,Vallone, B. (登録日: 2019-04-05, 公開日: 2020-03-11, 最終更新日: 2024-01-24)

主引用文献

Milazzo, L.,Exertier, C.,Becucci, M.,Freda, I.,Montemiglio, L.C.,Savino, C.,Vallone, B.,Smulevich, G.
Lack of orientation selectivity of the heme insertion in murine neuroglobin revealed by resonance Raman spectroscopy.
Febs J., 287:4082-4097, 2020

PubMed Abstract: Different murine neuroglobin variants showing structural and dynamic alterations that are associated with perturbation of ligand binding have been studied: the CD loop mutants characterized by an enhanced flexibility (Gly-loop and Gly-loop ), the F106A mutant, and the double Gly-loop /F106A mutant. Their ferric resonance Raman spectra in solution and in crystals are almost identical. In the high-frequency region, the identification of a double set of core size marker bands indicates the presence of two 6-coordinate low spin species. The resonance Raman data, together with the corresponding crystal structures, indicate the presence of two neuroglobin conformers with a reversed (A conformer) or a canonical (B conformer) heme insertion orientation. With the identification of the marker bands corresponding to each conformer, the data indicate that the B conformer increases at the expense of the A form, predominantly in the Gly-loop /F106A double mutant, as confirmed by X-ray crystallography. This is the first time that a reversed heme insertion has been identified by resonance Raman in a native 6-coordinate low-spin heme protein. This diagnostic tool could be extended to other heme proteins in order to detect heme orientational disorder, which are likely to be correlated to functionally relevant heme dynamics. DATABASE: Crystallographic structure: structural data are deposited in the Protein Data Bank under the 6RA6 PDB entry.

PubMed: 32034988
DOI: 10.1111/febs.15241

実験手法

X-RAY DIFFRACTION (2.3 Å)