6R7M

Tobacco Mosaic Virus (TMV)

6R7M の概要

• エントリーDOI: 10.2210/pdb6r7m/pdb
• EMDBエントリー: 4628
• 分子名称: Capsid protein (1 entity in total)
• 機能のキーワード: tobacco, mosaic, virus, tmv, cryo-em, cryowriter, microfluidic
• 由来する生物種: Tobacco mosaic virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17092.00

構造登録者

Schmidli, C.,Albiez, S.,Rima, L.,Righetto, R.,Mohammed, I.,Oliva, P.,Kovacik, L.,Stahlberg, H.,Braun, T. (登録日: 2019-03-29, 公開日: 2019-07-03, 最終更新日: 2024-05-15)

主引用文献

Schmidli, C.,Albiez, S.,Rima, L.,Righetto, R.,Mohammed, I.,Oliva, P.,Kovacik, L.,Stahlberg, H.,Braun, T.
Microfluidic protein isolation and sample preparation for high-resolution cryo-EM.
Proc.Natl.Acad.Sci.USA, 116:15007-15012, 2019

PubMed Abstract: High-resolution structural information is essential to understand protein function. Protein-structure determination needs a considerable amount of protein, which can be challenging to produce, often involving harsh and lengthy procedures. In contrast, the several thousand to a few million protein particles required for structure determination by cryogenic electron microscopy (cryo-EM) can be provided by miniaturized systems. Here, we present a microfluidic method for the rapid isolation of a target protein and its direct preparation for cryo-EM. Less than 1 μL of cell lysate is required as starting material to solve the atomic structure of the untagged, endogenous human 20S proteasome. Our work paves the way for high-throughput structure determination of proteins from minimal amounts of cell lysate and opens more opportunities for the isolation of sensitive, endogenous protein complexes.

PubMed: 31292253
DOI: 10.1073/pnas.1907214116

実験手法

ELECTRON MICROSCOPY (1.92 Å)