6R31

Family 11 Carbohydrate-Binding Module from Clostridium thermocellum in complex with beta-1,3-1,4-mixed-linked tetrasaccharide

6R31 の概要

• エントリーDOI: 10.2210/pdb6r31/pdb
• 関連するPDBエントリー: 1V0A
• 分子名称: Endoglucanase H, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: ctcbm11 beta-1, 3-1, 4-mixed-linked glucan clostridium thermocellum, sugar binding protein
• 由来する生物種: Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21052.94

構造登録者

Ribeiro, D.O.,Carvalho, A.L. (登録日: 2019-03-19, 公開日: 2020-02-05, 最終更新日: 2024-05-15)

主引用文献

Ribeiro, D.O.,Viegas, A.,Pires, V.M.R.,Medeiros-Silva, J.,Bule, P.,Chai, W.,Marcelo, F.,Fontes, C.M.G.A.,Cabrita, E.J.,Palma, A.S.,Carvalho, A.L.
Molecular basis for the preferential recognition of beta 1,3-1,4-glucans by the family 11 carbohydrate-binding module from Clostridium thermocellum.
Febs J., 287:2723-2743, 2020

PubMed Abstract: Understanding the specific molecular interactions between proteins and β1,3-1,4-mixed-linked d-glucans is fundamental to harvest the full biological and biotechnological potential of these carbohydrates and of proteins that specifically recognize them. The family 11 carbohydrate-binding module from Clostridium thermocellum (CtCBM11) is known for its binding preference for β1,3-1,4-mixed-linked over β1,4-linked glucans. Despite the growing industrial interest of this protein for the biotransformation of lignocellulosic biomass, the molecular determinants of its ligand specificity are not well defined. In this report, a combined approach of methodologies was used to unravel, at a molecular level, the ligand recognition of CtCBM11. The analysis of the interaction by carbohydrate microarrays and NMR and the crystal structures of CtCBM11 bound to β1,3-1,4-linked glucose oligosaccharides showed that both the chain length and the position of the β1,3-linkage are important for recognition, and identified the tetrasaccharide Glcβ1,4Glcβ1,4Glcβ1,3Glc sequence as a minimum epitope required for binding. The structural data, along with site-directed mutagenesis and ITC studies, demonstrated the specificity of CtCBM11 for the twisted conformation of β1,3-1,4-mixed-linked glucans. This is mediated by a conformation-selection mechanism of the ligand in the binding cleft through CH-π stacking and a hydrogen bonding network, which is dependent not only on ligand chain length, but also on the presence of a β1,3-linkage at the reducing end and at specific positions along the β1,4-linked glucan chain. The understanding of the detailed mechanism by which CtCBM11 can distinguish between linear and mixed-linked β-glucans strengthens its exploitation for the design of new biomolecules with improved capabilities and applications in health and agriculture. DATABASE: Structural data are available in the Protein Data Bank under the accession codes 6R3M and 6R31.

PubMed: 31794092
DOI: 10.1111/febs.15162

実験手法

X-RAY DIFFRACTION (2.6 Å)