6R2O
Hemoglobin structure from serial crystallography with a 3D-printed nozzle.
Summary for 6R2O
| Entry DOI | 10.2210/pdb6r2o/pdb |
| Descriptor | Hemoglobin subunit alpha, Hemoglobin subunit beta, PROTOPORPHYRIN IX CONTAINING FE, ... (7 entities in total) |
| Functional Keywords | hemoglobin, serial crystallography, lcls, oxygen transport |
| Biological source | Equus caballus (Horse) More |
| Total number of polymer chains | 4 |
| Total formula weight | 64718.32 |
| Authors | Oberthuer, D.,Yefanov, O.,Sarrou, I. (deposition date: 2019-03-18, release date: 2020-01-29, Last modification date: 2024-01-24) |
| Primary citation | Knoska, J.,Adriano, L.,Awel, S.,Beyerlein, K.R.,Yefanov, O.,Oberthuer, D.,Pena Murillo, G.E.,Roth, N.,Sarrou, I.,Villanueva-Perez, P.,Wiedorn, M.O.,Wilde, F.,Bajt, S.,Chapman, H.N.,Heymann, M. Ultracompact 3D microfluidics for time-resolved structural biology. Nat Commun, 11:657-657, 2020 Cited by PubMed Abstract: To advance microfluidic integration, we present the use of two-photon additive manufacturing to fold 2D channel layouts into compact free-form 3D fluidic circuits with nanometer precision. We demonstrate this technique by tailoring microfluidic nozzles and mixers for time-resolved structural biology at X-ray free-electron lasers (XFELs). We achieve submicron jets with speeds exceeding 160 m s, which allows for the use of megahertz XFEL repetition rates. By integrating an additional orifice, we implement a low consumption flow-focusing nozzle, which is validated by solving a hemoglobin structure. Also, aberration-free in operando X-ray microtomography is introduced to study efficient equivolumetric millisecond mixing in channels with 3D features integrated into the nozzle. Such devices can be printed in minutes by locally adjusting print resolution during fabrication. This technology has the potential to permit ultracompact devices and performance improvements through 3D flow optimization in all fields of microfluidic engineering. PubMed: 32005876DOI: 10.1038/s41467-020-14434-6 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.46 Å) |
Structure validation
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