6R2O

Hemoglobin structure from serial crystallography with a 3D-printed nozzle.

6R2O の概要

• エントリーDOI: 10.2210/pdb6r2o/pdb
• 分子名称: Hemoglobin subunit alpha, Hemoglobin subunit beta, PROTOPORPHYRIN IX CONTAINING FE, ... (7 entities in total)
• 機能のキーワード: hemoglobin, serial crystallography, lcls, oxygen transport
• 由来する生物種: Equus caballus (Horse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64718.32

構造登録者

Oberthuer, D.,Yefanov, O.,Sarrou, I. (登録日: 2019-03-18, 公開日: 2020-01-29, 最終更新日: 2024-01-24)

主引用文献

Knoska, J.,Adriano, L.,Awel, S.,Beyerlein, K.R.,Yefanov, O.,Oberthuer, D.,Pena Murillo, G.E.,Roth, N.,Sarrou, I.,Villanueva-Perez, P.,Wiedorn, M.O.,Wilde, F.,Bajt, S.,Chapman, H.N.,Heymann, M.
Ultracompact 3D microfluidics for time-resolved structural biology.
Nat Commun, 11:657-657, 2020

PubMed Abstract: To advance microfluidic integration, we present the use of two-photon additive manufacturing to fold 2D channel layouts into compact free-form 3D fluidic circuits with nanometer precision. We demonstrate this technique by tailoring microfluidic nozzles and mixers for time-resolved structural biology at X-ray free-electron lasers (XFELs). We achieve submicron jets with speeds exceeding 160 m s, which allows for the use of megahertz XFEL repetition rates. By integrating an additional orifice, we implement a low consumption flow-focusing nozzle, which is validated by solving a hemoglobin structure. Also, aberration-free in operando X-ray microtomography is introduced to study efficient equivolumetric millisecond mixing in channels with 3D features integrated into the nozzle. Such devices can be printed in minutes by locally adjusting print resolution during fabrication. This technology has the potential to permit ultracompact devices and performance improvements through 3D flow optimization in all fields of microfluidic engineering.

PubMed: 32005876
DOI: 10.1038/s41467-020-14434-6

実験手法

X-RAY DIFFRACTION (2.46 Å)