6R0J

The N-terminal domain of rhomboid protease YqgP

6R0J の概要

• エントリーDOI: 10.2210/pdb6r0j/pdb
• NMR情報: BMRB: 34376
• 分子名称: Rhomboid family serine protease (1 entity in total)
• 機能のキーワード: rhomboid protease, bacillus subtilis, metal binding, membrane protein
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21836.58

構造登録者

Began, J.,Strisovsky, K.,Veverka, V. (登録日: 2019-03-13, 公開日: 2020-01-08, 最終更新日: 2024-06-19)

主引用文献

Began, J.,Cordier, B.,Brezinova, J.,Delisle, J.,Hexnerova, R.,Srb, P.,Rampirova, P.,Kozisek, M.,Baudet, M.,Coute, Y.,Galinier, A.,Veverka, V.,Doan, T.,Strisovsky, K.
Rhomboid intramembrane protease YqgP licenses bacterial membrane protein quality control as adaptor of FtsH AAA protease.
Embo J., 39:e102935-e102935, 2020

PubMed Abstract: Magnesium homeostasis is essential for life and depends on magnesium transporters, whose activity and ion selectivity need to be tightly controlled. Rhomboid intramembrane proteases pervade the prokaryotic kingdom, but their functions are largely elusive. Using proteomics, we find that Bacillus subtilis rhomboid protease YqgP interacts with the membrane-bound ATP-dependent processive metalloprotease FtsH and cleaves MgtE, the major high-affinity magnesium transporter in B. subtilis. MgtE cleavage by YqgP is potentiated in conditions of low magnesium and high manganese or zinc, thereby protecting B. subtilis from Mn /Zn toxicity. The N-terminal cytosolic domain of YqgP binds Mn and Zn ions and facilitates MgtE cleavage. Independently of its intrinsic protease activity, YqgP acts as a substrate adaptor for FtsH, a function that is necessary for degradation of MgtE. YqgP thus unites protease and pseudoprotease function, hinting at the evolutionary origin of rhomboid pseudoproteases such as Derlins that are intimately involved in eukaryotic ER-associated degradation (ERAD). Conceptually, the YqgP-FtsH system we describe here is analogous to a primordial form of "ERAD" in bacteria and exemplifies an ancestral function of rhomboid-superfamily proteins.

PubMed: 31930742
DOI: 10.15252/embj.2019102935

実験手法

SOLUTION NMR